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KMID : 1007520110200030823
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Food Science and Biotechnology
2011 Volume.20 No. 3 p.823 ~ p.827
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Simultaneous Determination of Macrolide Residues in Fish and Shrimp by Liquid Chromatography-Tandem Mass Spectrometry
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Jo Mi-Ra
Lee Hee-Jung
Lee Tae-Seek
Park Kun-ba-wui
Oh Eun-Gyoung
Kim Poong-Ho
Lee Doo-Seog
Masakazu Horie
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Abstract
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A sensitive and selective analytical method for 7 macrolides determination by LC-tandem mass spectrometry (LC-MS/MS) in fish and shrimp was developed. Samples were extracted by liquid-liquid extraction steps using 70% acetonitrile. The positive ionization produced the molecular related ions, (M+2H)2+, at m/z 422.3 for spiramycin I, and (M+H)+, at m/z 734.5, 772.4, 828.4, 837.4, 916.5, and 814.4 for erythromycin A, kitasamycin, josamycin, roxithromycin, tylosin A, and oleandomycin, respectively, were the precursor ions for collision-induced dissociation (CID) and 2 diagnostic product ions for each analyte were identified to carry out selected reaction monitoring (SRM) LC-MS/MS analyses. The recovery rates of macrolides in fish and shrimp samples were in the range 68.2-89.0, 72.8- 91.6% and 75.2-87.0, 64.4-83.2% for the concentration of 50 and 100 ng/g, respectively.
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KEYWORD
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macrolide antibiotics, LC-tandem mass spectrometry (LC-MS/MS), fish, shrimp
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